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. Author manuscript; available in PMC: 2021 Oct 9.
Published in final edited form as: Sci Transl Med. 2020 Jul 15;12(552):eaaw5831. doi: 10.1126/scitranslmed.aaw5831

Fig. 5. Development of dose estimation algorithm, dose reconstruction, and validation.

Fig. 5.

(A) Dose estimation algorithms developed for each time point (green, blue, magenta, and turquoise for 24, 48, 96, and 168 hours after gamma irradiation, respectively) by plotting normalized ΔΔCt data points from 3-month-old C57BL/6 female mice. (B) Linear regression plots of absorbed dose estimated versus actual doses at 24 hours, (C) 48 hours, and (D) 96 hours generated using 48-hour algorithm and (E) 168 hours using 168-hour algorithm. For each plot, dose estimated from 5 to 10 blinded samples is given on the y axis, while actual exposed dose is given on the x axis. (F) Combined plot with data pooled from 24, 48, 96, and 168 hours (20 to 40 data points per dose). Lack-of-fit test P > 0.05 and R2 > 0.8. (G) Box plots show the deviations between the actual dose and the estimated dose (combined n = 20 to 40 data points per dose). (H) ROC curves demonstrate AUC values >0.9 at each time point for actual absorbed dose >2 or >6 Gy.