Figure 6.
Protein-protein interaction landscape of 3CLpro cell substrates
(A) Network of direct (solid line) and physically associated (dashed line) interactors (blue dots) of the 101 3CLpro substrates (red circles) generated by the Intact-database (accessed May 2021). Interactors of ≥ 2 substrates are in magenta, only interactors of ≥ 3 substrates are labeled, the number of substrate interactions is indicated by circle size. Orange circles, candidate substrates manually annotated.
(B) Top 5 CORUM protein complexes statistically enriched in the 3CLpro substrate-human interactome.
(C) One-step direct neighborhood protein-protein interaction network of 3CLpro human substrates (n = 74, red circles) that are directly connected to SARS-CoV-2 proteins (n = 26, yellow diamonds) or connected via direct neighbors (n = 197). Assembled from IMex/Intact Coronavirus dataset accessed June 2, 2021. Yellow diamonds, viral proteins. Blue circles, direct interactors connecting viral proteins to substrates. Magenta circles, direct interactors connecting viral proteins to ≥ 2 substrates. Black edge, SARS-CoV-2/human protein interactions.
See also Data S1.