Fig. 5.

WEE1 inhibition enhances the CD8⁺ T cell activation induced by MUS81 knockdown. a Representative images of CD8⁺ T cells activation after co-cultures. CD69⁺ cells were gated in CD3⁺/CD8⁺ T cells. MFC shScramble or shMUS81 cells were treated with dimethyl sulfoxide (DMSO) or MK1775 (1.0 μM). b Statistics analysis of the proportion of CD69⁺ cells to CD3⁺/CD8⁺ T cells in the indicated four groups. The data are presented as mean ± SD (n = 3). c–e ELISA analysis of TNF-α (left), IL-1β (middle), and IL-6 (right) in the supernatants of the co-cultures. The data are presented as mean ± SD (n = 3). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns., not significant