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. 2021 Oct 9;21:461. doi: 10.1186/s12870-021-03241-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Reliability of inducible expression in subsequent generations and GUS activity in rice. A Ten-day-old rice seedlings (line 65) of generation T1, T2 (A and B) and T3 (A1, A2 and B1, B2) histochemically stained for GUS activity induced by germinating and growing the seedlings on ½ MS plates containing 30 μM Dex or control DMSO (−Dex) for 10 days. Seedlings of pZmUbi::GUS were included as a control for the GUS staining. Scale bar represents 1 cm. B, C GUS activity determined fluorometrically in roots (B) and shoots (C) of 7-day-old rice seedlings (lines 65 and 121, T1 and T2) germinated and grown on ½ MS plates containing 30 μM Dex or control DMSO (−Dex) for 7 days. Seedlings of NT and pZmUbi::GUS were included as controls. The error bars represent SD