Fig. 3.

Time course accumulation of induced GUS activity and dose-response of the pOp6/LhGR system in rice. GUS activity determined fluorometrically in 7-day-old rice seedlings (lines 65B and 121C) germinated on ½ MS plates (vertically placed in the incubator) and then stood up in 15 ml Falcon tubes with 2 ml water for further 5 days. The newly developed leaves were induced by painting with water supplemented with 0.1% Tween-20 and (A) 10 μM Dex for 0, 6, 12, 24, 48 and 72 h or (B) with 0, 0.01, 0.1, 1 and 10 μM Dex for 48 h. Seedlings of NT and pZmUbi::GUS were included as controls. The error bars represent SD. C Roots of 10-day-old seedlings (lines 65A, 121C) were induced in liquid ½ MS with 30 μM Dex for 0, 12, 24. 48 and 72 h, then histochemically stained for GUS activity. Representative images are shown. D Roots of 10-day-old seedlings (lines 65A, 121C) were induced in liquid ½ MS for 24 h with 0, 1, 3, 10 and 30 μM Dex, then histochemically stained for GUS activity. Representative images are shown. Scale bar represents 1 cm