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. 2021 Oct 1;118(40):e2108239118. doi: 10.1073/pnas.2108239118

Fig. 4.

Fig. 4.

AAV-delivered Id2 induces functional synapse formation. (A) Electron microscopy images show ZnT3-positive boutons (red) on GC dendrite and spines (green) 3 mo after AAV-Id2. Insets a and b are shown enlarged, whereas c and d show additional examples that are not present in panel A. (B) 3D electron-tomographic reconstruction of a ZnT3-positive MF bouton located in the GCL/IML border 3 mo after AAV-Id2. Red: axon shaft, green: mitochondria, blue: synapse formed by this bouton terminal. (C) Experimental design and injection schedule to test physiological transmission after MF rewiring. In acute brain slices, patch-clamp recordings were made from ChR-negative GCs, while ChR-positive GCs were activated with blue light (∼30% of total GCs population in these experiments) 3 mo after AAV-ChR (Control) and AAV-Id2 (mixed with AAV-ChR) injections. (D) Cumulative probability plot shows the fraction of recorded cells versus light-evoked EPSC amplitude in GCs of dorsal hippocampus (recorded in 10 µM Gabazine; Kolmogorov-Smirnov test, P = 0.57). Inset shows light-evoked EPSC amplitudes (Mann–Whitney U test, *P = 0.048). (E) Cumulative probability plot shows the fraction of recorded cells versus light-evoked EPSC amplitude in GCs of ventral hippocampus (recorded in 10 µM Gabazine; Kolmogorov-Smirnov test, *P = 0.041). Inset shows light-evoked EPSC amplitudes (Mann–Whitney U test, **P = 0.0075).