Table 1.

RNAi gene knockdowns that suppress myo-3p::PR50-GFP toxicity

C. elegans	Yeast	Fly	Human	Description	Predicted or demonstrated NLS	Suppresses GR50?	Lethal/sterile
Nuclear transport/RNA binding
rsp-6	MRD1	X16*	SRSF3 †	RNA binding protein	Yes	Yes	Yes
ztf-4	NGR1	CG42458*	NCOA5/hnRNPC†	Contains RRM domain, similar to hnRNPC	Yes	Yes	Yes
gyf-1	SYH1	Gigyf	GIGYF †	Yeast homolog SYH1 binds proline-rich sequences and influences nuclear pore distribution	Yes	Yes	No
ima-3	KAP122‡	Kap-α3*,§	KPNA3 †	One of three nuclear importin alpha subunits	No	No	Yes
ergo-1	None	AGO1T1	AGO3	Production of endo-siRNAs	Yes	Yes	No
Ubiquitin-mediated protein degradation
spop-1	None	roadkill	SPOP	Speckle-type POZ protein; no yeast homolog	Yes	Yes	No
ufd-2	Ufd2	UBE4B/ CG9934	UBE4a	E3 and/or E4 ubiquitin ligase	Yes	Yes	No
rpn-12	RPN12	RPN12	PSMD8 ¶	nonATPase regulatory subunit of the 26S proteasome	No	Yes	No
rpn-9	RPN9	RPN9	PSMD13	nonATPase regulatory subunit of the 26S proteasome	No	Yes	Yes
Chromatin regulation
met-2	MET2	eggless	SETDB1	Histone methyltransferase	Yes	Yes	Yes
utx-1	CYC8	Utx	UTX/UTY #	Histone demethylase	Yes	Yes	Yes
pis-1	None	PTIP	PAXIP1	Pax transcription factor interacting protein	Yes	No	Yes

^*Enhanced the toxicity of GR50 in Drosophila model (19).

^† Interacts with both PR and GR in human cells (19).

^‡ Suppresses the toxicity of PR50 when overexpressed in yeast (10).

^§ Enhanced the toxicity of PR25 in Drosophila model (22).

^¶ Gene is enriched or depleted in PR20-treated K562 cell CRISPR screen (20).

^# Gene is enriched or depleted in GR20-treated K562 cell CRISPR screen (20).