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AP fluorometry of Cys engineered CaV1.1 with two cysteine reacting dyes attached to VSDII. Exemplar AP-induced fluorometric recordings from a fiber expressing EGFP-CaV1.1 L522C (VSDII) and stained with TAMRA (A) or TMRM (B) in control (gray traces) or BTS-added (red traces) recording solution. (C) TAMRA signal from a fiber not expressing a Cys-engineered CaV1.1. No early AP-evoked fluorescence is observed in labeled fibers expressing EGFP-CaV1.1 without engineered Cys.
