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. 2021 Oct 9;18:201. doi: 10.1186/s12985-021-01672-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Effects of CCL21 and Poly (I:C) as adjuvants on specific IgG antibody levels. Columns represent relative antibody expression in each treated group (n = 3). The measured values for influenza-specific total IgG (a), IgG1 (b), and IgG2a (c) in immune sera were reported as the mean ± SD, and One way ANOVA is used to calculate the statistical significance. Differences between all treatment groups and control groups were statistically high significant (***p < 0.001). The highest level of total IgG and IgG1 isotype was also observed in mice primed with γ-Flu plus CCL21-Poly (I:C) while there was no significant difference among the groups immunized with γ-Flu-CCL21, γ-Flu-Poly (I:C), or γ-Flu-CCL21-Poly (I:C) in the number of IgG2a secreting cells