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. 2021 Sep 16;49(18):10630–10643. doi: 10.1093/nar/gkab774

Figure 2.

Figure 2.

PABPN1 facilitates ZFC3H1 to its targets. (A) The illustration of possible roles of CBC and PABPN1 in ZFC3H1 recruitment. (B) Western blot to examine the KD efficiency of ARS2 and CBP80. GAPDH was used as a loading control. The white line delineates the boundary where irrelevant lanes have been removed from the same blot. (C, D) RNA-IP analysis to examine the association of ZFC3H1 with SNHG RNAs upon CBC KD. IPs were carried out with a ZFC3H1 antibody in cells treated with Cntl or CBC siRNAs, followed by RT-qPCRs to detect RNAs (C) and western blot to detect ZFC3H1 (D). Error bars, standard deviations (n = 3). The white line delineates the boundary where irrelevant lanes have been removed from the same blot. (E) Western blot to examine the KD efficiency of PABPN1. GAPDH was used as a loading control. The white line delineates the boundary where irrelevant lanes have been removed from the same blot. (F, G) Same as (C) and (D), except that PABPN1 KD cells were used instead of CBC KD cells. Error bars, standard deviations (n = 3). Statistical analysis was performed using Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant.