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Prime editing using an all-in-one plasmid vector in HEK293T cells. (A) Schematic representation of the PEA1 plasmid, containing three BbsI golden gate assembly sites to insert the customizable guide target sequences and RT template. (B) PEA1 editing efficiencies of targeted 1- and 3-bp insertions, 1- and 3-bp deletions and point mutations at four genomic sites in HEK293T cells. Mean ± SD, n = 3.
