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. 2021 Sep 23;49(18):10644–10656. doi: 10.1093/nar/gkab782

Figure 5.

Figure 5.

SprY and RNAIII ratio in different media. (A) HG003 wild-type strain was grown in BHI at 37°C. RNA was extracted at different times of growth. sprY and rnaIII expression profiles were analyzed by northern blot. (B) Analysis of sprY and rnaIII expressions in different media. HG003 wild-type strain was grown in different media (BHI, TSB, NZM and RPMI). Total RNA was extracted at 2, 6 and 10 h of growth and studied by northern blot using labeled DNA probes for SprY and RNAIII, and tmRNA as loading control. (C) The quantification of SprY and RNAIII by qPCR absolute. The RNA samples were prepared from the pellet of HG003 wild-type strain in BHI, TSB, NZM and RPMI at 2 and 6 h of growth. The chart represents the ratio SprY/RNAIII in these media. The error bars correspond to the average values from three independent experiments.