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CRISPR-Cas9 based validation of REMS in HUVECs. (A–C) Genomic position of the considered REMs (shown in black) and the area targeted by the gRNAs (shown in grey). These REMs are predicted to be involved in the regulation of the genes KLF2 (A), AC020916 (B) and NOS3 (C). (D) Comparison between RNA levels for control and CRISPR-Cas9 knock-outs in HUVEC clones (3 replicates). Statistical significance was assessed with a one-sided t-test.
