Table 3.

Summary for Interaction Changes of Mutations Based on Structural Analysis

Interaction Changes	Mutations
No interaction with RIF	P45S, T400I, K446Q, I488V, G759S, Q1056K
No change	Q429H, S431G, D435G
Hydrogen bond lost	Q432P, Q432L, H445R, H445L, H445Y, H445C, H445D, H445P, H445N, H445Q, S450L, S450F, S450G
Hydrophobic interaction lost	L430P, L430R, L452P, I491N, I491V
Steric hindrance	V170F, Q432L, D435V, D435E, D435Y, S441L, H445Y, S450L, S450F, L452P, I491M
Electrostatic repulsion	Q432K