Fig. 1.

Design and development of a rMIKO-1 protein and its three derivatives. (A) The full-length amino acid (AA) sequences of rS100A8 and rS100A9 were schematically drawn by open and closed horizontal bars, respectively. (B) rMIKO-1: 24 AA residues from the C-terminal AA residue of rS100A9 were added to the C-terminal AA residue of rS100A8. rMIKO-2 and rMIKO-3: 20 AA residues from the N-terminal AA residue of rS100A8 and those of rS100A9 were replaced. rMIKO-4: 24 AA residues, as indicated by the dotted line, from the C-terminal AA residue of rS100A9 were deleted. (C) SDS-PAGE (15% gel) was performed using affinity-purified proteins in the presence of 2-mercaptoethanol. M: molecular weight markers. Lanes 1 to 6 indicate purified rMIKO-1, -2, -3, -4, rS100A8, and rS100A9, respectively.