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. 2021 Oct 9;12(10):924. doi: 10.1038/s41419-021-04234-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Sorting of gastric cancer stem-like cells. The fluorescence activated cell sorting was performed based on the detection of ALDH1 activity using the ALDH1 fluorescent substrate BODIPY-aminoacate (BAAA). As a control, the activity of ALDH1 was inhibited by DEAB. The ALDH1-positive cells were potential gastric cancer stem-like cells (D region) and ALDH1-negative cells were cancer non-stem-like cells (C region). b Tumorsphere formation assay. The ALDH1-positive cells were subjected to tumorsphere formation assay. The sphere formation was examined with a light microscope. Scale bar, 100 μm. c Tumorigenicity of cancer stem-like cells in nude mice. Mice were subcutaneously injected with ALDH1-positive or ALDH1-negative cells. Forty days later, the tumors were examined. Arrows indicate the tumors. d Differential expressions of stemness-associated genes in gastric cancer stem-like cells and non-stem-like cells. Quantitative real-time PCR was conducted to detect the mRNA levels (^**p < 0.01). e Tumorsphere formation of gastric cancer stem-like cells from the solid tumors of patients with gastric cancer. Scale bar, 10 μm. f The expression levels of stemness genes in gastric cancer stem-like cells isolated from the solid tumors of gastric cancer patients. The statistical significance of difference between treatments was indicated with asterisks (^**p < 0.01). g Tumorigenicity of the potential gastric cancer stem-like cells in mice. Nude mice were subcutaneously injected with the potential gastric cancer stem-like cells or gastric cancer non-stem-like cells. Forty days later, the tumors were examined. Arrows indicate the tumors.