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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Glia. 2021 Aug 20;69(12):2882–2898. doi: 10.1002/glia.24075

Figure 4: Hey1 and Id2b regulate MG’s regenerative response.

Figure 4:

A, Illustration of hey1 gene promoter from human, mouse and zebrafish with Rbpj binding sites indicated. B, Luciferase assays for Notch (NICD) dependent activation of Wt and mutant hey1 promoter activity; Compared to Wt alone, ***P<0.0001. C, F, EdU Click-iT chemistry on retinal sections identifies proliferating cells in Wt fish treated with indicated MOs (C) or Cas9 and indicated gRNAs (F). D, G, I, Quantification of the number of EdU+ cells at 4 dpi; (D)***P=0.0008, (G) ***P<0.0001, (I) ***P<0.0001. E, H, Quantification of the width of the proliferative zone in retinas at 4 dpi; (E) ***P<0.0001, (H) ***P=0.0004. J, PCR analysis of the effects Hey1 expression has on injury-dependent induction of ascl1a and lin28a. Abbreviations: MO, morpholino; C-MO or Con-MO, control-MO; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; Wt, wild type; m, mutant. Scale bars are 100 μm.