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. Author manuscript; available in PMC: 2022 Aug 10.
Published in final edited form as: Neurosci Lett. 2021 Jun 12;759:136048. doi: 10.1016/j.neulet.2021.136048

Figure 1. Spectrogram of mouse pup ultrasonic vocalizations.

Figure 1.

Spectrogram generated from P5-P7 pup isolation calls using Avisoft SASLab Pro software. Four individual vocalizations are shown in the representative spectrogram. The horizontal axis shows time (in seconds) from which duration of each USV could be determined as well as a total duration of vocalization during the 5-minute testing period. The vertical axis shows frequency (kHZ) of vocalizations emitted by mouse pups. The amplitude of calls (dB) can also be determined by the color (scale shown in top bar).