Fig. 1.

Temporal trajectory of scRNA-seq profiles during development of the sea urchin Lytechinus variagatus. (A) A timeline of development over 24 h, with representative stages of development illustrated. Colors represent lineages as in B. (B) UMAP plot mapped according to lineage domains. Sixty-three clusters were identified and grouped into 16 domains representing the lineages present at 24 hpf. (C) The UMAP plot colored according to time of development showing the position of the 18 time points collected during the first 24 h post-fertilization (hpf). PGC, primordial germ cells; CP, coelomic pouch; PMC, primary mesenchyme cell (also called skeletogenic cells); NSM, non-skeletogenic mesenchyme; Pigment, pigment cells; Blasto, blastocoelar cells; Endomeso, endomesoderm; Endo, endoderm; Ecto, ectoderm; Border, ectoderm at the border between ectoderm and endoderm; OR, oral; CB, ciliary band; APD, animal pole domain; AB, aboral.