Figure 1.

Experimental design. A, Cyanotoxin exposure plan for zebrafish from 6 hpf to 6 dpf. B, High-throughput behavior testing apparatus: multi-well testing grid is mounted on an acoustic shaker above an infrared (IR) LED array and below an IR-sensitive high-speed camera. A white LED is mounted above the grid to simulate daylight conditions. Videos are analyzed with automated tracking software (FLOTE). C, Proteomics workflow: zebrafish larvae from the mixture exposed groups were pooled for protein extraction and tryptic digestion of extracted proteins into peptides. nLC-MS/MS label-free protein quantitation via MaxQuant statistical analysis via Perseus software and enrichment analysis via ingenuity pathway analysis (IPA).