Figure 5.

Examples of 2 patterns of Lactobacillus crispatus CTV-05 colonization following LACTIN-V administration in the phase 2b trial. A, Two women successfully colonized with L. crispatus CTV-05 throughout the 24-week duration of the study and (B) 2 women successfully colonized with L. crispatus CTV-05 only during LACTIN-V dosing through week 12. Vaginal pH was measured and vaginal swabs were collected at screening, prior to metronidazole treatment (day −1), at enrollment, after metronidazole and before LACTIN-V treatment (day 0), and then at weeks 4, 8, 12 (during treatment) and at 24 (13 weeks post treatment). DNA was extracted from vaginal swabs. Primers were designed for quantitative polymerase chain reaction to amplify pan 16S rRNA for total bacteria, and specific primers for all Lactobacillus species, L. crispatus species, and L. crispatus CTV-05 strain.