Table I.
Summary of library statistics
| CR6261 Full Library | CR6261 Full Library | CR9114 Full Library | CR9114 Full Library | |||||
|---|---|---|---|---|---|---|---|---|
| Replicate 1 | Replicate 2 | Replicate 1 | Replicate 2 | |||||
| CR6261 | UCA_CR6261 | CR6261 | UCA_CR6261 | CR9114 | UCA_CR9114 | CR9114 | UCA_CR9114 | |
| Sequencing reads after quality filtering (Fold coverage) | 4 639 427 (283) | 1 133 491 (69) | 2 750 816 (168) | 1 332 944 (81) | 1 373 400 (42) | 3 504 280 (107) | 2 044 245 (62) | 2 247 779 (69) |
| Percentage of reads: | ||||||||
| Wildtype parental DNA | 4.74% | 0.42% | 2.28% | 0.47% | 0.04% | 0.06% | 0.03% | 0.49% |
| Encoded mutations | 74.24% | 74.28% | 73.85% | 75.58% | 76.06% | 65.13% | 78.33% | 77.22% |
| Desired mutations | 55.18% | 52.28% | 53.96% | 54.86% | 40.47% | 32.88% | 39.11% | 40.50% |
| Library coverage (Number of variants made) | 95.89% (15711/16384) | 97.52% (15978/16384) | 96.72% (15846/16384) | 98.81% (16189/16384) | 97.56% (31967/32768) | 98.85% (32390/32768) | 95.20% (31194/32768) | 86.24% (28260/32768) |
| Overall library coverage | 99.93% (16 373/16 384) | 99.99% (16 382/16 384) | 99.97% (32 757/32 768) | 99.03% (32 451/32 768) | ||||