Table 2.

Correlations between age and area of immunoreactivity for steroidogenic enzymes

	All (N = 60)		Men (N = 30)		Women (N = 30)
	r	P-value	r	P-value	r	P-value
Adrenal cortex area	0.34	0.0008	0.41	0.02	0.29	0.12
CYP11B2-positive area	−0.50	<0.0001	−0.67	<0.0001	−0.31	0.09
VSNL1-positive area	−0.42	0.0007	−0.57	0.001	−0.34	0.06
HSD3B2-positive area	0.35	0.007	0.34	0.07	0.35	0.06
CYB5A-positive area	−0.76	<0.0001	−0.74	<0.0001	−0.77	<0.0001
CYP11B1-positive area	0.02	0.86	0.11	0.56	−0.06	0.75
Estimated ZF area	0.53	<0.0001	0.59	0.0006	0.49	0.007
Ratio of CYP11B2 per VSNL1 area	−0.31	0.02	−0.47	0.009	−0.11	0.57

All enzymatic areas are represented as % of total cortical area. The estimated ZF proportion was calculated by subtracting the % VSNL1-positive area from the % HSD3B2-positive area. Spearman’s rank correlation coefficients were used to evaluate the associations between age and each parameter.

Abbreviations: CYB5A, cytochrome b₅ type A; CYP11B1, 11β-hydroxylase; CYP11B2, aldosterone synthase; HSD3B2, 3β-hydroxysteroid dehydrogenase type II; VSNL1, Visinin-like 1; ZF, zona fasciculata.