Table 3.
Detection of Clarithromycin Susceptibility Testing by E-Test, Sequencing and the Multiplex qPCR of 23S rDNA
| No. of Samples | Culture | E-Testb | Sequencingb (No. of Samples) | qPCR of ureA | Multiplex qPCR of 23S rDNAc (No. of Samples) | Median of Bacterial Load (Bacteria) |
|---|---|---|---|---|---|---|
| 38 | Positive | Sensitive | T2182 (34) WTa (4) |
Positive | WTa (38) | 103 |
| 21 | Positive | Resistance | A2142G (2) A2143G (19) |
Positive | A2142G (2) A2143G (19) |
102 103 |
| 70 | Negative | Negative | Negative | Positive | WTa (70) | 103 |
| 7 | Negative | Negative | Negative | Positive | A2142G (1) A2142G + WTa (4) A2143G + WTa (2) |
102 102 (A2142), 103 (WT) 102 (A2143), 103 (WT) |
Notes: aNo mutation at nucleotide position 2142 and 2143. bE-test and sequencing were performed on H. pylori-positive isolates by culture (59 clinical isolates). cMultiplex qPCR of 23S rDNA was performed on H. pylori-positive samples by qPCR of ureA (136 biopsy samples).