Table 4.
Performance of the Multiplex qPCR of 23S rDNA and Multiplex qPCR of gyrA Assays for Detection of Clarithromycin and Levofloxacin Resistance in Biopsy Samplesa
| No. of Biopsies with the Following Result: | Sp (%) (95% Cl) | Se (%) (95% Cl) | PPV (95% Cl) | NPV (95% Cl) | Accuracy (%) | Kappa | P (McNemar) | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| TP | TN | FP | FN | ||||||||
| Clarithromycin resistance | 21 | 542 | 7 | 0 | 98.7 (97.3–99.4) | 100 (80.8–100) | 75 (54.8–88.6) | 100 (99.1–100) | 98.8 | 0.851 | 0.016 |
| Levofloxacin resistance | 15 | 554 | 1 | 0 | 99.8 (98.9–99.9) | 100 (74.7–100) | 93.8 (67.7–99.7) | 100 (99.1–100) | 99.8 | 0.967 | 1.000 |
Notes: aTrue-positive (TP) biopsies were defined as biopsies resistant by both the qPCR and E-test. True-negative (TN) biopsies were defined as biopsies susceptible by both the qPCR and E-test. False-positive (FP) biopsies were defined as biopsies susceptible by E-test and resistant by the qPCR. False-negative (FN) biopsies were defined as biopsies susceptible by qPCR and resistant by E-test.
Abbreviations: Sp, specificity; Se, sensitivity; PPV, positive predictive value; NPV, negative predictive value.