Table 5.
Detection of Levofloxacin Susceptibility Testing by E-Test and Sequencing to the Multiplex qPCR of gyrA
| No. of Samples | Culture | E-Testd | Sequencingd (No. of samples) | qPCR of ureA | Multiplex qPCR gyrAe (No. of Samples) | Median of Bacterial Load (Bacteria) |
|---|---|---|---|---|---|---|
| 44 | Positive | Sensitive | WTa (44) | Positive | N87WTb+D91WTc (44) | 105 (N87WT), 106 (D91WT) |
| 15 | Positive | Resistance | N87I (2) N87K (8) D91N (2) D91Y (3) |
Positive | N87K (1) N87WTb+D91N (2) N87WTb+D91Y (2) N87K+D91Y (1) N87K+D91WTc (5) N87I+D91WTc (2) N87WTb+N87K+D91WTc (2) |
101 105 (N87WT), 105 (D91N) 101 (N87WT), 106 (D91Y) 101 (N87K), 106 (D91Y) 104 (N87K), 107 (D91WT) 104 (N87I), 106 (D91WT) 104 (N87WT), 103 (N87K), 106 (D91WT) |
| 76 | Negative | Negative | Negative | Positive | N87WTb+D91WTc (76) | 105 (N87WT), 106 (D91WT) |
| 1 | Negative | Negative | Negative | Positive | N87K +D91WTc (1) | 101 (N87K), 105 (D91WT) |
Notes: aNo mutation at amino acid position 87 and 91; bNo mutation at amino acid position 87; cNo mutation at amino acid position 91. dE-test and sequencing were performed on H. pylori-positive isolates by culture (59 clinical isolates). eMultiplex qPCR of gyrA was performed on H. pylori-positive samples by qPCR of ureA (136 biopsy samples).