Figure 3.

Overexpression of PUM1 promoted prostate cancer cell proliferation and colony formation.

A: Western blotting analysis of PUM1 protein expression from cells overexpressing PUM1 or PUM1 and shPUM1 at the same time. B: Cell proliferation assay on PUM1 overexpression and PUM1 knockdown. Data are presented as mean±SD (two-tailed Student's t-test; ^**P<0.01,^***P<0.001). C: Colony assays were performed on the effect of overexpression ofPUM1 or knockdown of PUM1 on colony number and size. Scale bar: 100 μm. D: Colony number was counted for cells containing PUM1 overexpressing plasmids or knockdown plasmid, or both. Data are mean±SD of three independent experiments (Student's t-test; ^*P<0.05,^**P<0.01).