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. 2021 Jul 16;35(5):371–382. doi: 10.7555/JBR.35.20210067

Figure 5.

Figure 5

PUM1 repressed the translation of CDKN1B mRNA by binding to the PBE motif on its 3′ UTR.

A: Boxplots showed the expression of CDKN1B between normal prostate (n=52) and prostate carcinoma (n=497). Data were obtained from the GEPIA database (http://gepia.cancer-pku.cn/). B: The correlation analysis of PUM1 and CDKN1B in prostate cancer using the GEPIA tool. C: qRT-PCR analysis of CDKN1B mRNA levels in DU145 and PC3 cells with PUM1 knockdown. Data are expressed as mean±SD. Statistical analyses were performed by two-tailed Student's t-test for comparisons between each two groups. D–F: Cell cycle proteins expression levels in PC3, DU145, and LNCaP cells after 72 hours transfected with shPUM1-1 or shCTL were detected by Western blotting analysis. G: Western blotting analysis of CDKN1B protein level from DU145 cells transfected with human PUM1 (PUM1OE) or empty plasmids. TPM: transcripts per million.