Figure 4.

Depletion of SNX5 induced the instability of VAChT.

A: HeLa cells were transiently transfected with specific SNX5 siRNA or scrambled siRNA for 24 hours. SNX5 protein level in the cell lysates was detected with anti-SNX5 antibody by Western blotting. B: Half-life of VAChT expression was examined in control and SNX5-deleted HeLa cells after CHX (100 μg/mL) treatment at the indicated time points. VAChT expression level was detected with anti-VAChT antibody by Western blotting and quantified by densitometry analysis. The plots represent the mean of 3 independent experiments (^*P<0.05,^**P<0.01; unpaired, two-tailedt-test, SNX5 siRNA vs. Control; error bars, standard error of the mean). CHX: cycloheximide.