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. 2021 Oct 11;8:225. doi: 10.1038/s41438-021-00701-0

Fig. 3. Overexpression of MdWRKY75e conferred enhanced resistance to B. cinerea infection in transgenic tobacco.

Fig. 3

a Time course of B. cinerea infection of WT, V#12, and MdWRKY75e transgenic lines (#9, #32, and #36) for 48 h for phenotype analysis. b Time course of B. cinerea infection of WT, V#12, and MdWRKY75e transgenic lines for 48 h for disease index analysis. c, d Phenotypes of plants (c) and root (d) systems in tobacco WT, V#12, and MdWRKY75e transgenic lines. e, f Results of leaf (e) and root (f) dry weight measurements in tobacco WT, V#12, and MdWRKY75e transgenic lines. g–i Expression of NtLAC7 (g), activity of laccase (h), and lignin content of the leaves (i) in tobacco WT, V#12, and MdWRKY75e transgenic lines after 0 and 24 h of B. cinerea infection. j Root lignin content in tobacco WT, V#12, and MdWRKY75e transgenic lines. WT: wild-type; V#12: empty vector; and MdWRKY75e#9, MdWRKY75e#32, and MdWRKY75e#36: MdWRKY75e transgenic lines