Skip to main content
. 2021 Oct 11;8:225. doi: 10.1038/s41438-021-00701-0

Fig. 6. Silencing of MdWRKY75e by virus-induced gene silencing (VIGS) resulted in improved infection sensitivity in apple.

Fig. 6

a Semiquantitative RT–PCR detection of MdWRKY75e gene silencing efficiency. b–d Time course of the expression of the MdWRKY75e (b), MdLAC7 (c), and MdWFRK1 (d) genes in WT, pTRV2-GFP, pTRV2, and pTRV2-MdWRKY75e at 24 h. e Time course of A. alternata infection of WT, pTRV2-GFP, pTRV2, and pTRV2-MdWRKY75e for 48 h for phenotype analysis. f Time course of A. alternata infection of WT, pTRV2-GFP, pTRV2, and pTRV2-MdWRKY75e for 48 h for disease index analysis. g, h Time course of the expression of Alta1 (g) and GAPdh (h) in WT, pTRV2-GFP, pTRV2, and pTRV2-MdWRKY75e after A. alternata infection for 48 h. i, j Laccase activity (i) and leaf lignin content (j) in WT, pTRV2-GFP, pTRV2, and pTRV2-MdWRKY75e at 0 and 24 h after A. alternata infection