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. 2021 Sep 27;12:729524. doi: 10.3389/fphar.2021.729524

FIGURE 5.

FIGURE 5

Inhibition of Fascin-1 expression using siRNA in BV-2 microglia can be partly rescued by treatment with myelin. (A) Western blot analysis shows that compared to the nonspecific control (NC), Fascin-1 siRNA (siFascin-1) could knockdown the expression of Fascin-1, while myelin treatment could upregulate the expression of Fascin-1. (B) Quantitative analysis of Fascin-1 expression in (A). GAPDH was used as the loading control. The blots (n = 4 per group) were quantified as previously described. The results are expressed as the mean ± SEM. * p < 0.05 (NC + Myelin or siFascin-1 vs NC); # p < 0.05 (NC + Myelin vs siFascin-1), ### p < 0.001 (siFascin-1+Myelin vs siFascin-1). (C) BV-2 cells were transfected with nonspecific control (NC) siRNA or siFascin-1 for 24 h and then treated with or without myelin for an additional 24 h in complete medium. Representative immunofluorescence images of Fascin-1 (Green) and Iba1 (Red) are shown. DAPI (Blue) was used to stain the nuclei. Scale bar: 100 μm.