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. 2021 Sep 27;12:729524. doi: 10.3389/fphar.2021.729524

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Copyright © 2021 Yu, Cheng, Tian, Li, Yao, Luo, Liu, Zhu, Zheng and Jing.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Microglial polarization has no effect on Fascin-1 expression. (A) Western blot analysis shows the protein expression of M1-like (iNOS) or M2-like (CD206) polarization markers and Fascin-1 in BV-2 cells after polarization treatment. (B) Quantitative analysis of Fascin-1 expression in (A). GAPDH was used as the loading control. The blots (n = 5 per group) were quantified, as previously described, and no significant difference was observed. (C,D) Representative immunofluorescence images of M1-like (iNOS, Green) or M2-like (CD206, Green) markers and Fascin-1 (Red) in BV-2 cells after polarization treatment. The results also indicated no obvious differential expression of Fascin-1. DAPI (Blue) was used to stain the nuclei. Scale bar: 50 μm.