Figure 3.

Effect of isoform-specific PKD siRNA on phosphorylation of various MAPKs induced by LPA in MASMCs. The specific PKD siRNAs (40 nM) were transfected into MASMCs for 48 h followed by serum starvation for 24 h; then cells were stimulated with 10 μM of LPA for 5 min. A, effect of knockdown of the expression of PKD isoforms with PKD siRNAs on LPA-induced MAPK phosphorylation. Expression of PKDs and phosphorylation of MAPKs were examined by Western blot analysis. B, results were quantified by densitometry. Data are mean ± SD from three experiments. ∗∗p < 0.01 versus LPA-treated, nonsilencing siRNA group.