Figure 4.

Effects of the specific p38 MAPK inhibitor, the specific JNK MAPK inhibitor, and the specific MEK1/2 inhibitor on LPA-induced TF expression in MASMCs. The effect of the specific MEK1/2 inhibitor on LPA-induced phosphorylation of JNK and p38 MAPK was also examined. MASMCs were pretreated with the specific inhibitors for 45 min, then stimulated with LPA for 5 h for detection of TF expression. To determine MAPK phosphorylation, cell lysates of MASMCs stimulated by LPA for 5 min after pretreatment of specific ERK inhibitor were collected. TF expression and MAPK phosphorylation were examined by Western blot analysis. A, effect of various doses of the specific p38 MAPK inhibitor SB203580 on LPA-induced TF expression. B, results were quantified by densitometry. Data are mean ± SD from three experiments. ∗p < 0.05 and ∗∗p < 0.01 versus LPA only group. C, effect of various doses of the specific JNK inhibitor SP600125 on LPA-induced TF expression. D, results were quantified by densitometry. Data are mean ± SD from three experiments. ∗p < 0.05 and ∗∗p < 0.01 versus LPA only group. E, effect of combination of SP600125 and SB203580 on LPA-induced TF expression. F, results were quantified by densitometry. Data are mean ± SD from three experiments. ∗p < 0.05 and ∗∗p < 0.01 versus LPA only group. G, effect of various doses of the specific MEK1/2 inhibitor U0126 on LPA-induced TF expression. H, results were quantified by densitometry. Data are mean ± SD from three experiments. ∗p < 0.05 and ∗∗p < 0.01 versus LPA only group. I, effect of various doses of the specific MEK1/2 inhibitor U0126 on LPA-induced phosphorylation of JNK and p38 MAPK. J, results were quantified by densitometry. Data are mean ± SD from three experiments. ∗∗p< 0.01 versus LPA only group. K, effect of combination of U0126 and SB203580 on LPA-induced TF expression. L, results were quantified by densitometry. Data are mean ± SD from three experiments. ∗p < 0.05 and ∗∗p< 0.01 versus LPA only group.