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Advancements in image acquisition methods and resolution, enable the study of NVU component interactions as shown here by the interaction between MG endfeet (blue) and blood vessels (magenta), separated by the BM (white arrowhead; inset) in the developing zebrafish retina. The image was acquired with Zeiss LSM 900 AiryScan2 microscopy that allows in vivo acquisition with a resolution of 120 × 120 × 350 nm (x,y,z).
