Fig. 5.

GSTP-linked S-glutathionylaton of Pyruvate Kinase M2 attenuates its glycolysis activity. A, Total PSSG in lung tissue from saline- and HDM-exposed WT and Gstp^−/- mice, analyzed by DTNB assay. n=9–10 per group. B, Representative Western blot of PKM2-SSG in saline vs HDM-exposed mouse lungs. C, Pyruvate kinase enzymatic activity assay of 10 ng recombinant PKM2 incubated with 50 μM oxidized glutathione (GSSG). D, Complex models and interactions between dimeric or tetrameric PKM2 with a GSTP dimer by protein-protein docking. PDB structures used for modeling: 1T5A (PKM2 tetramer) and 1AQW (GSTP dimer). The most likely models are illustrated here, and all predicted models are shown in a cartoon in the supplemental information. E, Representative western blots of GSTP-PKM2 interaction via co-immunoprecipitation and total PKM2 levels in IL-1β-treated mouse lung tissues. F, Representative Western blot of PKM2-GSTP (IP: PKM2; IB; GSTP) and total PKM2 levels in wild type MTE cells pre-treated with 50 μM TLK199 for 1 h followed by stimulation with 1 ng/ml IL-1β for 2 h *p < 0.05 analyzed by two-way ANOVA.