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. 2021 Sep 27;9:716610. doi: 10.3389/fcell.2021.716610

FIGURE 3.

FIGURE 3

Knocking down sonic Hedgehog (SHH) in osteoarthritis mesenchymal stromal cell (OA-MSC) inhibited proliferation and gene expression of markers of chondrogenesis, catabolism, and senescence. (A) Significant inhibition of SHH mRNA levels by SHH-siRNA (siSHH) transfection in primary OA-MSC. NC: normal control with mock transfection. p-value is indicated (n = 3). (B) Significant inhibition of OA-MSC cell proliferation by siSHH in a dosage-dependent (50 and 75 nmol) manner. After transfection of siSHH for 48 h, the OA-MSC proliferation rate was quantified by CCK-8 proliferation assay. siSHH transfection significantly inhibited SOX9, MMP13, and ADAMTS5 (C) and p21 (D) in primary OA-MSC. (E) Significant inhibition of SHH mRNA levels by siSHH transfection in OA-MSC cell line. NC: normal control with mock transfection. p-value is indicated (n = 3). siSHH transfection significantly inhibited SOX9, MMP13, and ADAMTS5 (F) and p21 (G) in OA-MSC cell line. All data are reported as mean ± S.E.M (n = 3). p-value is indicated.