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. 2021 Sep 27;12:750827. doi: 10.3389/fgene.2021.750827

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Copyright © 2021 Wang, Dong, Wang, Jiang, Zhang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differentially expressed miRNA analysis and miRNA-mRNA potential interaction network construction. For the miRNA-seq dataset GSE159508, limma package was used to analyze the DEMs between the Group induced and the Group Control. A volcano plot (A) and heat map (B) shows the 16 DEMs. Red color indicates six up-regulated miRNA, and blue indicates 10 down-regulated miRNA. The predicted target genes of DEMs is calculated by miRWalk website. After the predicted target genes of DEMs and the obtained hub genes were intersected, a negatively regulated PPI of miRNA-mRNA network was obtained (C,D). We obtained five up-regulated (miR-337-3p, miR-376c-3p, miR-4288, miR-483-5p, and miR-654-3p) and 6 down-regulated (miR-25-5p, miR-3940-5p, miR-584-5p, miR-642b-3p, miR-663a, and miR-874-3p) DEMs that potentially interacted to hub genes. Group Control: PDLSCs treated with control medium. Group induced: PDLSCs treated with osteogenic medium.