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. 2021 Sep 27;12:750827. doi: 10.3389/fgene.2021.750827

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Copyright © 2021 Wang, Dong, Wang, Jiang, Zhang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiR-584-5p regulates the osteogenic differentiation of PDLSCs in vitro. PDLSCs were transfected with inhibitor NC, miR584-5p inhibitor, mimics NC and miR584-5p mimics. After 24 h of transfection, the relative expression of miR584-5p was detected by qPCR (A). Cells were cultured in osteogenic induction medium for 7, 14 days. ALP staining (7 days) and Alizarin Red staining (14 days) were used to detect ALP activity and the formation of mineralized nodules, respectively (B). Osteogenesis related indicators ALPL, BMP2, RUNX2 and OCN were used to further prove the osteogenic regulation of miR-584-5p (C,D). **p < 0.01 and ***p < 0.001.