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. 2021 Sep 27;12:744527. doi: 10.3389/fendo.2021.744527

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Copyright © 2021 Lucas, Tencerova, von der Weid, Andersen, Attané, Behler-Janbeck, Cawthorn, Ivaska, Naveiras, Podgorski, Reagan and van der Eerden

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic protocol for isolation of BMAds and BMSCs. (A) Strategy to recover BMAds and BMSCs depending of the tissue source. (B) Wash and mince the selected tissue. (C) Digestion strategy using collagenase digestion to increase the release and the purity of BMAds. Some encountered challenges are shown such as the lysis (1) and low yield (2) of BMAds. At the end of the process pure BMAds are obtained. (D) BMSCs isolation with tubes showing the variation observed between samples. BMSCs are obtained after centrifugation of the serum fraction and the BSMC-enriched fraction.