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. 2021 Sep 27;12:744527. doi: 10.3389/fendo.2021.744527

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Copyright © 2021 Lucas, Tencerova, von der Weid, Andersen, Attané, Behler-Janbeck, Cawthorn, Ivaska, Naveiras, Podgorski, Reagan and van der Eerden

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro evaluation of BMSC quality (BMSC culture, CFU-C, tri-differentiation). (A) Representative picture of spindle-like shape cells in in vitro culture of human BMSCs. (B) CFU-F of BMSCs after 14 days in vitro culture visualized by Violet Blue in a petri dish. (C) Alizarin staining of mineralized matrix in 10 days osteoblast (OB) culture differentiated from primary BMSCs. (D) Oil Red O staining of neutral lipids in a 10 days adipocyte (AD) culture differentiated from primary BMSCs. (E) Alcian Blue staining of a 21 days chondrocyte (CH) culture differentiated from BMSCs. Scale bar represents 200 μm in all the pictures.