Table 2.

Differential stabilities for individual framework point mutants.

VH Gene	Mutation (paper)	Mutation (IMGT)	Minimum nt	Score	Property*	Reference
VH3-23	Q81H	Q90H	1	16.1	Mean ΔTm = 0.0°C‡	(48)
	A84P	A96P	1	15.1	ΔT50 = 0.7°C	(49)
VH1-69	M48G	M53G	2	-1.4	ΔT50 = 3°C	(50)
	M48I	M53I	1	11.4	ΔT50 = 2°C
	V67I	V76I	1	14.6	ΔT50 = 4°C
	V67L	V76L	1	19.1	ΔT50 = 7°C
VH6-1	V72D	V69D	2	10.5	320% a , 180% b yield	(51)
					0.1 a , 2.2 b kJ/mol stability
	S76G	S74G	1	26.8	210% a , 150% b yield
					3.7 a , 3.5 b kJ/mol stability
	S90Y	S88Y	1	4.4	180% a , 230% b yield
					-0.1 a , 1.4 b kJ/mol stability

^‡Mean ΔT_m was calculated over all genotypes presented in Table 3 in ref (48).

^*Yield is calculated as a relative percentage to the original antibody sequence.

^aAntibody 2C2.

^bAntibody 6B3.