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. 2021 Sep 27;11:711735. doi: 10.3389/fonc.2021.711735

Figure 7.

Figure 7

OTUB2 promotes GC stem cell properties through regulating KDM1A expression. (B) After MKN-45 cells were co-transfected with OTUB2 overexpression plasmids/NC and shKDM1A/Scr. The HGC-27 cells were co-transfected with shOTUB2/shNC and OV-KDM1A/vector, the cell viability was detected by MTT assay (A); The spheroid-forming ability of GC cells were detected by sphere formation assay (B). Wound healing assays (C) and Transwell assays (D) were used to determine the changes in migratory and invasive abilities of GC cells; Relative protein levels of KDM1A, Nanog, ALDH, and Oct4 IN GC cells were measured by Western blot (E). Data was represented as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001. # P < 0.05, ## P < 0.01, and ### P < 0.001.