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. 2021 Oct 5;2(4):100863. doi: 10.1016/j.xpro.2021.100863

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© 2021 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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CSC speed determination from kymographs in Fiji

(A) First image in the time-lapse movie.

(B) The sum intensity projection of all images in the time-lapse movie. Line segment in magenta is overlaying a chosen CSC track for speed analysis and was copied to the original time-lapse movie in (A).

(C) Kymograph generated from the magenta line segment in (A). The yellow line represents a single CSC trajectory chosen for speed measurement.

(D) Kymograph of a SmaCC particle, marked with white arrows. SmaCCs are usually bright and move erratically. Scale bars in A and B: 20 μ, scale bars in C and D: 2 μm. See also Supplemental Dataset 1.