Single-cell RNA-Seq analysis of chondrocytes isolated from PFE cartilage of Cre and CreTW mice.A, experimental workflow of scRNA-Seq analysis. B, summary of cell numbers, reads, identified genes, and count of filtered UMIs mapped to each barcode per genotype (barcode rank plots). C and D, PCA-based t-distributed stochastic neighbor embedding (t-SNE) plot of merged (C) and single datasets of Cre- and CreTW-derived cells (D). Colors indicate UMI counts (C) or genotype (D). PCA, principal component analysis; PFE, proximal femoral epiphysis; UMI, unique molecular identifier.