Figure 4.

Identification of genes regulated between chondrocyte subpopulations of Cre and CreTW mice.A, the transcriptome was compared between articular, prehypertrophic/hypertrophic, and unique subpopulation of PFE cartilage from 1-month-old mice using Loupe software. Regulated mtDNA-encoded genes are marked (square). mtDNA-encoded genes commonly regulated in all subpopulations (red arrowhead), and ECM-related genes are depicted (black arrowheads). B, Log2 expression intensities of regulated mtDNA-encoded genes are indicated (color) in t-SNE plots of single cells from Cre or CreTW mice. C, subpopulation-specific log2 expression values are given (color) in split t-SNE plots of single cells for the mtDNA-encoded mt-Nd4l gene and the nuclear-encoded ECM-related genes. ECM, extracellular matrix; mtDNA, mitochondrial DNA; PFE, proximal femoral epiphysis; t-SNE, t-distributed stochastic neighbor embedding.