Table 1.
List of most abundant mitochondrial proteins identified by LC–MS/MS in the flavin fluorescent gel bands F1 and F2 resolved by hrCNE from Figure 1
Number | Protein name | Molecular weight (kDa) | Access number (National Centerfor Biotechnology Information) | Protein spectrum counta |
|
---|---|---|---|---|---|
F1 | F2 | ||||
1 | 2-oxoglutarate dehydrogenase | 118.18 | Q60597-3 | 415 | 6 |
2 | Dihydrolipoyllysine-succinyltransferase | 48.99 | Q9D2G2 | 277 | 1 |
3 | OGDLH protein | 114.54 | B2RXT3_MOUSE | 234 | 3 |
4 | MICOS complex subunit Mic60 | 82.93 | Q8CAQ8-2 | 234 | 70 |
5 | ADP/ATP translocase 2 | 32.93 | ADT2_MOUSE | 90 | 32 |
6 | Dihydrolipoyl dehydrogenase | 54.27 | DLDH_MOUSE | 53 | 5 |
5 | NADH-ubiquinone oxidoreductase 75 kDa | 79.70 | Ndufs1 | 33 | 328 |
6 | ATP synthase subunit beta | 59.75 | Atp5f1b | 79 | 242 |
7 | NADH dehydrogenase flavoprotein 1 | 49.91 | Ndufv1 | 18 | 112 |
8 | NADH dehydrogenase FeS protein 2 | 52.63 | Ndufs2 | 20 | 107 |
9 | NADH dehydrogenase subunit 10 | 40.60 | Ndufa10 | 15 | 93 |
10 | NADH dehydrogenase subunit 9 | 42.12 | Ndufa9 | 16 | 71 |
Protein spectrum count was normalized by the total spectral count. The detailed MS data are provided in Table S1.