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Comparison of wild-type human ADO and the C18S/C239S variant by gel filtration chromatography. The chromatogram was the result of gel filtration chromatography using a Superdex 200 column with 50 mM HEPES, 50 mM NaCl (pH 7.5). Black and red traces are for wild-type hADO and the C18S/C239S variant, respectively.
