Table 1.
Composition of the experimental diets, g/kg (on as-fed basis).
| NaCl |
NaHCO3 |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| PC | NC1+0 FTU/kg | NC1+500 FTU/kg | NC1+1,000 FTU/kg | NC1+2,000 FTU/kg | NC2+0 FTU/kg | NC2+500 FTU/kg | NC2+1,000 FTU/kg | NC2+2,000 FTU/kg | |
| Diet description1 | A | B | C | D | E | F | G | H | I |
| Ingredients, g/kg | |||||||||
| Corn | 484.2 | 452.5 | 452.5 | 452.5 | 452.5 | 451.7 | 451.7 | 451.7 | 451.7 |
| Soybean meal, 48% | 391.7 | 390.0 | 390.0 | 390.0 | 390.0 | 390.1 | 390.1 | 390.1 | 390.1 |
| Soybean oil | 60.0 | 60.0 | 60.0 | 60.0 | 60.0 | 60.0 | 60.0 | 60.0 | 60.0 |
| Limestone | 12.5 | 12.2 | 12.2 | 12.2 | 12.2 | 12.2 | 12.2 | 12.2 | 12.2 |
| Dicalcium phosphate | 15.7 | 10.4 | 10.4 | 10.4 | 10.4 | 10.4 | 10.4 | 10.4 | 10.4 |
| NaCl | 3.92 | 2.93 | 2.93 | 2.93 | 2.93 | 1.39 | 1.39 | 1.39 | 1.39 |
| NaHCO3 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 2.25 | 2.25 | 2.25 | 2.25 |
| Vitamin mineral premix2 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 |
| DL-Methionine | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 |
| L-Lysine HCl | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 |
| Phytase premix3 | 0.00 | 0.00 | 10.00 | 20.00 | 40.00 | 0.00 | 10.00 | 20.00 | 40.00 |
| Ground corn | 0.00 | 40.00 | 30.00 | 20.00 | 0.00 | 40.00 | 30.00 | 20.00 | 0.00 |
| Titanium dioxide premix4 | 25 | 25 | 25 | 25 | 25 | 25 | 25 | 25 | 25 |
| Total | 1,000 | 1,000 | 1,000 | 1,000 | 1,000 | 1,000 | 1,000 | 1,000 | 1,000 |
| Calculated value, g/kg | |||||||||
| Calcium | 10.04 | 8.60 | 8.60 | 8.60 | 8.60 | 8.60 | 8.60 | 8.60 | 8.60 |
| Non-phytate P | 4.50 | 3.50 | 3.50 | 3.50 | 3.50 | 3.50 | 3.50 | 3.50 | 3.50 |
| Phytase activity, FTU/kg5 | < 50 | < 50 | 580 | 1,280 | 1,930 | < 50 | 416 | 1,060 | 2,800 |
PC, positive control; NC, negative control.
Vitamin-mineral premix was formulated to supply the following at 2.5 grams per kilogram of diet: 11 025 IU of vitamin A; 3,528 IU of vitamin D; 33 IU of vitamin E; 0.91 mg of vitamin K; 2.21 mg of thiamin; 7.72 mg of riboflavin; 55 mg of niacin; 18 mg of pantothenate; 5 mg of vitamin B-6; 0.22 mg d-biotin; 1.10 mg of folic acid; 478 mg of choline; 0.03 of vitamin B-12; 75 mg of Zn; 40 mg of Fe; 64 mg of Mn; 10 mg of Cu; 1.85 mg of I; and 0.30 mg of Se.
Phytase (Quantum Blue 5G, AB Vista) premix was formulated to supply 500 FTU/kg of diet when added to the diet at the rate of 10 g/kg. It was added to the diets at the expense of ground corn.
Prepared as 5 g of titanium dioxide mixed with 20 g of ground corn.
Analyzed value.